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VEDALAB Inc elisa hfsh kit
Elisa Hfsh Kit, supplied by VEDALAB Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Enzyme-linked Immunosorbent Assay:

Article Title: The Use of Electronic Sensor in Hormone Analysis
Article Snippet: .. The same sample was reanalyzed with ELISA hFSH Kit from Veda Lab and the results from both techniques were compared. ..



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<t>FSH</t> induces neuroinflammation in mice. Four weeks after the FSH treatment, the brain and serum of the mice were harvested for western blot (a,b), <t>Elisa</t> assay (c), and immunofluorescence staining (d,e). (a) Representative immunoblots showing the protein expression of IL-1β and IL-6 in the hippocampus. (b) Quantification of the immunoreactivity of IL-1β and IL-6 protein expression, data are shown as represent mean ± SEM, n = 4 mice per group. (c) ELISA quantification shows the levels of proinflammation factors IL-1β, IL-6, and TNFα in the serum, data are shown as mean ± SEM, n = 6 mice per group. (d) Immunofluorescent co-staining of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm). (e) Quantification of Iba1 positive and GFAP positive cells, data represent mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, * *p < 0.01; ns, not significant.
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a, Western immunoblots showing the effect of activating neuronal FSHRs by <t>FSH</t> (30 ng/mL) in human SH-SY5Y and primary rat neuronal cells on the expression of C/EBPβ, AEP, as well as the cleavage of amyloid precursor protein (APP) and Tau using antibodies noted in ‘Methods’. FSH (30 ng/mL) likewise stimulated the expression of CEBPB, LGMN, APP and MAPT (qPCR) (b); AEP activity (c); and certain inflammatory cytokines <t>(ELISA),</t> namely IL-6 and IL-1β (d). Statistics: mean ± s.e.m.; Mice per group, (b) 3, (c) 6, and (d) 6; one-way ANOVA. e, Western immunoblotting showing C/EBPβ, AEP, APP, cleaved APP1–585, total Tau, and cleaved Tau1–368 in response to FSH or PBS following transfection with human FSHR siRNA (si-FSHR) for SH-SY5Y cells or rat Fshr siRNA (si-Fshr) for primary rat neurons, or appropriate scrambled siRNAs. f, mRNA levels of CEBPB, LGMN, APP and MAPT in SH-SY5Y cells incubated with FSH after control or si-FSHR transfection. g, AEP activity after incubation with FSH in control or si-FSHR-transfected SH-SY5Y cells. h, IL-6 and IL-1β levels (ELISAs) in SH-SY5Y cells incubated with FSH following control or si-FSHR infection. Statistics: mean ± s.e.m.; (f) 3 biological replicates; (g, h) 6 mice per group; one-way ANOVA.
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Effects of platinum chemotherapeutic drugs on the levels <t>of</t> <t>gonadotropin,</t> melatonin, and oxidative stress in the peripheral blood of male gastrointestinal tumor patients, and reducing the expression of MT1/MT2 in the testis. ( A ) Human testicular tissue was stained with H&E (scale bar = 50 μm). ( B ) Images of TUNEL staining in human testicular tissue (scale bar = 50 μm). ( C ) Representative immunofluorescent pictures of MT1 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT1 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( D ) Representative immunofluorescent pictures of MT2 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT2 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( E ) MT1 mRNA expression in human testicular tissue. ( F ) MT2 mRNA expression in human testicular tissue. ( G ) GnRH level in human serum. ( H ) <t>FSH</t> level in human serum. ( I ) LH level in human serum. ( J ) Testosterone level in human serum. ( K ) Melatonin level in human serum. ( L ) Content of MDA in human serum. ( M ) SOD activity in human serum. ( N ) Total antioxidant capacity in human serum. Data are presented as the mean ± SEM. N = 3 cases per group in ( A – F ). N = 30 cases per group in ( G – N ). CT, chemotherapy. * p < 0.05, ** p < 0.01 versus the control group or the tumor group.
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Chemotherapy-induced premature ovarian insufficiency (POI) mice model. A Vaginal exfoliative cell smear and ovary section of normal mice (a–d, × 100; i, × 40; j, × 200) and POI mice (e–h, × 100; k, × 40, l, × 200). B Comparison of the follicles and the proportion of atretic follicles between CTX/BUS+ group and CTX/BUS− groups (* P < 0.05). C E2 and <t>FSH</t> levels determined by ELISA 14 days after CTX/BUS administration (* P < 0.05). E2 oestradiol, FSH follicle-stimulating hormone, CTX/BUS+ CTX/BUS administration, CTX/BUS− CTX/BUS non-administration
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Chemotherapy-induced premature ovarian insufficiency (POI) mice model. A Vaginal exfoliative cell smear and ovary section of normal mice (a–d, × 100; i, × 40; j, × 200) and POI mice (e–h, × 100; k, × 40, l, × 200). B Comparison of the follicles and the proportion of atretic follicles between CTX/BUS+ group and CTX/BUS− groups (* P < 0.05). C E2 and <t>FSH</t> levels determined by ELISA 14 days after CTX/BUS administration (* P < 0.05). E2 oestradiol, FSH follicle-stimulating hormone, CTX/BUS+ CTX/BUS administration, CTX/BUS− CTX/BUS non-administration
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Chemotherapy-induced premature ovarian insufficiency (POI) mice model. A Vaginal exfoliative cell smear and ovary section of normal mice (a–d, × 100; i, × 40; j, × 200) and POI mice (e–h, × 100; k, × 40, l, × 200). B Comparison of the follicles and the proportion of atretic follicles between CTX/BUS+ group and CTX/BUS− groups (* P < 0.05). C E2 and <t>FSH</t> levels determined by ELISA 14 days after CTX/BUS administration (* P < 0.05). E2 oestradiol, FSH follicle-stimulating hormone, CTX/BUS+ CTX/BUS administration, CTX/BUS− CTX/BUS non-administration
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FSH induces neuroinflammation in mice. Four weeks after the FSH treatment, the brain and serum of the mice were harvested for western blot (a,b), Elisa assay (c), and immunofluorescence staining (d,e). (a) Representative immunoblots showing the protein expression of IL-1β and IL-6 in the hippocampus. (b) Quantification of the immunoreactivity of IL-1β and IL-6 protein expression, data are shown as represent mean ± SEM, n = 4 mice per group. (c) ELISA quantification shows the levels of proinflammation factors IL-1β, IL-6, and TNFα in the serum, data are shown as mean ± SEM, n = 6 mice per group. (d) Immunofluorescent co-staining of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm). (e) Quantification of Iba1 positive and GFAP positive cells, data represent mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, * *p < 0.01; ns, not significant.

Journal: Frontiers in Molecular Neuroscience

Article Title: Follicle-stimulating hormone induces depression-like phenotype by affecting synaptic function

doi: 10.3389/fnmol.2024.1459858

Figure Lengend Snippet: FSH induces neuroinflammation in mice. Four weeks after the FSH treatment, the brain and serum of the mice were harvested for western blot (a,b), Elisa assay (c), and immunofluorescence staining (d,e). (a) Representative immunoblots showing the protein expression of IL-1β and IL-6 in the hippocampus. (b) Quantification of the immunoreactivity of IL-1β and IL-6 protein expression, data are shown as represent mean ± SEM, n = 4 mice per group. (c) ELISA quantification shows the levels of proinflammation factors IL-1β, IL-6, and TNFα in the serum, data are shown as mean ± SEM, n = 6 mice per group. (d) Immunofluorescent co-staining of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm). (e) Quantification of Iba1 positive and GFAP positive cells, data represent mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, * *p < 0.01; ns, not significant.

Article Snippet: Recombinant human FSH protein (hor-249) was from Prospecbio; antibody to IL-6 (YT5348) was from Immuneway; antibodies to IL-1 β (16806-1-AP), GluR1 (67642-1-Ig), FSHR (22665-1-AP), GAD67 (10408-1-AP), VGluT1 (21829-1-AP), and synaptophysin (17785-1-AP) were from Proteintech; antibodies to GluR2 (A11316), synapsin (WX620330), PSD95 (A7889), ERK1/2 (A4782), and phospho-ERK1/2 (AP0472) were from Abclonal; antibody to GFAP (AB-2532994) was from Invitrogen; antibody to Iba1 (019-19741) was from Wako; antibody to β-actin (GB11001-100) was from Servicebio; ERK1/2 inhibitor (HY-112287) was from MedChemExpress; IL-1β mouse ELISA kit (MU30369), IL-6 mouse ELISA kit (MU30044), and TNF- α mouse ELISA kit (MU30030) were from Bioswamp; human FSH ELISA kit (CSB-E06867h) and luteinizing hormone (LH) ELISA kit (CSB-E12770m) were from Cusabio; 17β-estradiol ELISA kit (ab108667) was from Abcam; 4′,6-diamidino-2-phenylindole (DAPI, D9542) was from Sigma-Aldrich; AAV9 sh-FSHR (BC-2403) and AAV9 sh-Control (BC-0183) adenovirus were customized, synthesized, and purified by Shenzhen Brain Case Biotechnology Inc.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing

FSHR knockdown alleviates FSH-induced neuroinflammation in mice. (a,b) Representative immunoblots images (a) and quantification (b) of IL-1β and IL-6 protein expression showing a reduction in the hippocampus of the sh-FSHR + FSH group compared to sh-Control + FSH group, data are presented as mean ± SEM, n = 4 mice per group. (c) ELISA quantification of pro-inflammation factors IL-1β, IL-6, and TNFα in the serum, data are presented as mean ± SEM, n = 6 mice per group. (d,e) Immunofluorescent co-staining (d) and quantification (e) of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm), data are presented as mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05, * *p < 0.01.

Journal: Frontiers in Molecular Neuroscience

Article Title: Follicle-stimulating hormone induces depression-like phenotype by affecting synaptic function

doi: 10.3389/fnmol.2024.1459858

Figure Lengend Snippet: FSHR knockdown alleviates FSH-induced neuroinflammation in mice. (a,b) Representative immunoblots images (a) and quantification (b) of IL-1β and IL-6 protein expression showing a reduction in the hippocampus of the sh-FSHR + FSH group compared to sh-Control + FSH group, data are presented as mean ± SEM, n = 4 mice per group. (c) ELISA quantification of pro-inflammation factors IL-1β, IL-6, and TNFα in the serum, data are presented as mean ± SEM, n = 6 mice per group. (d,e) Immunofluorescent co-staining (d) and quantification (e) of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm), data are presented as mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05, * *p < 0.01.

Article Snippet: Recombinant human FSH protein (hor-249) was from Prospecbio; antibody to IL-6 (YT5348) was from Immuneway; antibodies to IL-1 β (16806-1-AP), GluR1 (67642-1-Ig), FSHR (22665-1-AP), GAD67 (10408-1-AP), VGluT1 (21829-1-AP), and synaptophysin (17785-1-AP) were from Proteintech; antibodies to GluR2 (A11316), synapsin (WX620330), PSD95 (A7889), ERK1/2 (A4782), and phospho-ERK1/2 (AP0472) were from Abclonal; antibody to GFAP (AB-2532994) was from Invitrogen; antibody to Iba1 (019-19741) was from Wako; antibody to β-actin (GB11001-100) was from Servicebio; ERK1/2 inhibitor (HY-112287) was from MedChemExpress; IL-1β mouse ELISA kit (MU30369), IL-6 mouse ELISA kit (MU30044), and TNF- α mouse ELISA kit (MU30030) were from Bioswamp; human FSH ELISA kit (CSB-E06867h) and luteinizing hormone (LH) ELISA kit (CSB-E12770m) were from Cusabio; 17β-estradiol ELISA kit (ab108667) was from Abcam; 4′,6-diamidino-2-phenylindole (DAPI, D9542) was from Sigma-Aldrich; AAV9 sh-FSHR (BC-2403) and AAV9 sh-Control (BC-0183) adenovirus were customized, synthesized, and purified by Shenzhen Brain Case Biotechnology Inc.

Techniques: Knockdown, Western Blot, Expressing, Control, Enzyme-linked Immunosorbent Assay, Staining

a, Western immunoblots showing the effect of activating neuronal FSHRs by FSH (30 ng/mL) in human SH-SY5Y and primary rat neuronal cells on the expression of C/EBPβ, AEP, as well as the cleavage of amyloid precursor protein (APP) and Tau using antibodies noted in ‘Methods’. FSH (30 ng/mL) likewise stimulated the expression of CEBPB, LGMN, APP and MAPT (qPCR) (b); AEP activity (c); and certain inflammatory cytokines (ELISA), namely IL-6 and IL-1β (d). Statistics: mean ± s.e.m.; Mice per group, (b) 3, (c) 6, and (d) 6; one-way ANOVA. e, Western immunoblotting showing C/EBPβ, AEP, APP, cleaved APP1–585, total Tau, and cleaved Tau1–368 in response to FSH or PBS following transfection with human FSHR siRNA (si-FSHR) for SH-SY5Y cells or rat Fshr siRNA (si-Fshr) for primary rat neurons, or appropriate scrambled siRNAs. f, mRNA levels of CEBPB, LGMN, APP and MAPT in SH-SY5Y cells incubated with FSH after control or si-FSHR transfection. g, AEP activity after incubation with FSH in control or si-FSHR-transfected SH-SY5Y cells. h, IL-6 and IL-1β levels (ELISAs) in SH-SY5Y cells incubated with FSH following control or si-FSHR infection. Statistics: mean ± s.e.m.; (f) 3 biological replicates; (g, h) 6 mice per group; one-way ANOVA.

Journal: Nature

Article Title: FSH blockade improves cognition in mice with Alzheimer’s disease

doi: 10.1038/s41586-022-04463-0

Figure Lengend Snippet: a, Western immunoblots showing the effect of activating neuronal FSHRs by FSH (30 ng/mL) in human SH-SY5Y and primary rat neuronal cells on the expression of C/EBPβ, AEP, as well as the cleavage of amyloid precursor protein (APP) and Tau using antibodies noted in ‘Methods’. FSH (30 ng/mL) likewise stimulated the expression of CEBPB, LGMN, APP and MAPT (qPCR) (b); AEP activity (c); and certain inflammatory cytokines (ELISA), namely IL-6 and IL-1β (d). Statistics: mean ± s.e.m.; Mice per group, (b) 3, (c) 6, and (d) 6; one-way ANOVA. e, Western immunoblotting showing C/EBPβ, AEP, APP, cleaved APP1–585, total Tau, and cleaved Tau1–368 in response to FSH or PBS following transfection with human FSHR siRNA (si-FSHR) for SH-SY5Y cells or rat Fshr siRNA (si-Fshr) for primary rat neurons, or appropriate scrambled siRNAs. f, mRNA levels of CEBPB, LGMN, APP and MAPT in SH-SY5Y cells incubated with FSH after control or si-FSHR transfection. g, AEP activity after incubation with FSH in control or si-FSHR-transfected SH-SY5Y cells. h, IL-6 and IL-1β levels (ELISAs) in SH-SY5Y cells incubated with FSH following control or si-FSHR infection. Statistics: mean ± s.e.m.; (f) 3 biological replicates; (g, h) 6 mice per group; one-way ANOVA.

Article Snippet: The human FSH ELISA kit was obtained from Abcam (ab108641).

Techniques: Activation Assay, Western Blot, Expressing, Activity Assay, Enzyme-linked Immunosorbent Assay, Transfection, Incubation, Control, Infection

a, Ovariectomized 3xTg mice displayed hypoplastic thread-like uteri and elevated serum FSH and LH levels. FSH-Ab (200 μg/mouse, every 2 days, i.p., 8 weeks) did not alter total serum FSH, LH or 17β-estradiol levels. Statistics: mean ± s.e.m., N = 8 mice per group, one-way ANOVA. b, Immunofluorescent micrographs showing enhanced labelling in the following pairs: amyloid β (Aβ, red) and thioflavin-S (Thio-S, green); Aβ (red) and cleaved APPC586 (green); and pTau (red) and Tau1–368 (green) in the hippocampus after OVX, and its amelioration with FSH-Ab (scale bar, 20 μm). c, Upregulation of Cebpb, Lgmn, App and Mapt in OVX mouse brains, with reversal to near-baseline with FSH-Ab. Statistics: mean ± s.e.m., N = 3 mice per group, one-way AVOVA. d, Immunofluorescence micrographs showing that OVX induces apoptosis (TUNEL, green) in hippocampal NeuN-positive neurons (red); this apoptosis is abolished by FSH-Ab (scale bar, 20 μm). e, Golgi staining on brain sections from CA1 region post-OVX shows a substantial reduction in spine numbers, which is corrected with the FSH-Ab (scale bar, 5 μm). Statistics: mean ± s.e.m., N = 3 mice per group (10 sections), one-way AVOVA. f, Transmission electron micrographic images and quantitative analysis of synapses in hippocampal sections post-OVX treated with IgG or FSH-Ab (scale bar, 1 μm). Statistics: mean ± s.e.m., N = 3 mice per group (8 sections), one-way AVOVA. g, Morris Water Maze testing shows no differences in swim speed. Statistics: mean ± s.e.m., N = 9 mice per group, one-way ANOVA. h, Cognitive testing using the Novel Object Recognition test revealed the absence of significant difference between APP/PS1 and non-transgenic mice in Discrimination Index [(Novel Object Head Entry – Familiar Object Head Entry)/Total Head Entry]; the result is expected at 9 months of age in APP/PS1 mice. Thus, no effect of FSH-Ab was noted at this age, despite the reduction in Aβ40 and Aβ42 accumulation shown in Fig. 1f. Statistics: mean ± s.e.m., mice per group 9, 10, 4 and 4 from left to right; Whisker plot, upper and lower ends of the whiskers show maxima and minima, line in box shows median, and upper and lower box boundaries show 75th and 25th percentile, respectively; unpaired two-tailed Student’s t-test; i, ELISA showing no cross-reactivity of FSH-Ab with LH. j, Western immunoblot showing no change in expression of the LHCGR in whole brain lysates upon OVX or FSH-Ab treatment (N = 2 mice per group). k, IVIS imaging of isolated tissues from mice injected with AlexaFluor750–FSH, i.v., showing localization of FSH in the brain (N = 3 mice per group). l, Western immunoblots of whole brain lysates showing that i.p. injection of human FSH (5 IU) causes an elevation of brain FSH (N = 3 mice per group). m, Immunofluorescence micrographs showing the detection of peripherally injected (i.p.) biotinylated FSH-Ab (red) and biotinylated goat IgG (red) in brain sections (scale bar, 20 μm). Note the absence of cellular or nuclear co-localization with MAP2 or DAPI, respectively. n, Representative PET image shows that 89Zr-labelled humanized monoclonal FSH-Ab (89Zr-Hu6), injected i.v., is localized to live brain (arrows). γ-counting in perfused tissue shows presence of 89Zr-Hu6 in dissected brain tissue at 24 and 48 h post-injection (N = 4 mice). o, IVIS imaging and quantitation with AlexaFluor750-labelled Hu6, given i.v. shows localization in perfused whole brain tissue; N = 3 mice per group. Control (Ctrl): phosphate-buffered saline (PBS). p, Confirmatory immunofluorescence on the same mice (o) using anti-human IgG showing Hu6 localization (red) in proximity to CD31+ endothelial cells (green) (scale bar, 100 μm). For gel source data, see Supplementary Fig. 1.

Journal: Nature

Article Title: FSH blockade improves cognition in mice with Alzheimer’s disease

doi: 10.1038/s41586-022-04463-0

Figure Lengend Snippet: a, Ovariectomized 3xTg mice displayed hypoplastic thread-like uteri and elevated serum FSH and LH levels. FSH-Ab (200 μg/mouse, every 2 days, i.p., 8 weeks) did not alter total serum FSH, LH or 17β-estradiol levels. Statistics: mean ± s.e.m., N = 8 mice per group, one-way ANOVA. b, Immunofluorescent micrographs showing enhanced labelling in the following pairs: amyloid β (Aβ, red) and thioflavin-S (Thio-S, green); Aβ (red) and cleaved APPC586 (green); and pTau (red) and Tau1–368 (green) in the hippocampus after OVX, and its amelioration with FSH-Ab (scale bar, 20 μm). c, Upregulation of Cebpb, Lgmn, App and Mapt in OVX mouse brains, with reversal to near-baseline with FSH-Ab. Statistics: mean ± s.e.m., N = 3 mice per group, one-way AVOVA. d, Immunofluorescence micrographs showing that OVX induces apoptosis (TUNEL, green) in hippocampal NeuN-positive neurons (red); this apoptosis is abolished by FSH-Ab (scale bar, 20 μm). e, Golgi staining on brain sections from CA1 region post-OVX shows a substantial reduction in spine numbers, which is corrected with the FSH-Ab (scale bar, 5 μm). Statistics: mean ± s.e.m., N = 3 mice per group (10 sections), one-way AVOVA. f, Transmission electron micrographic images and quantitative analysis of synapses in hippocampal sections post-OVX treated with IgG or FSH-Ab (scale bar, 1 μm). Statistics: mean ± s.e.m., N = 3 mice per group (8 sections), one-way AVOVA. g, Morris Water Maze testing shows no differences in swim speed. Statistics: mean ± s.e.m., N = 9 mice per group, one-way ANOVA. h, Cognitive testing using the Novel Object Recognition test revealed the absence of significant difference between APP/PS1 and non-transgenic mice in Discrimination Index [(Novel Object Head Entry – Familiar Object Head Entry)/Total Head Entry]; the result is expected at 9 months of age in APP/PS1 mice. Thus, no effect of FSH-Ab was noted at this age, despite the reduction in Aβ40 and Aβ42 accumulation shown in Fig. 1f. Statistics: mean ± s.e.m., mice per group 9, 10, 4 and 4 from left to right; Whisker plot, upper and lower ends of the whiskers show maxima and minima, line in box shows median, and upper and lower box boundaries show 75th and 25th percentile, respectively; unpaired two-tailed Student’s t-test; i, ELISA showing no cross-reactivity of FSH-Ab with LH. j, Western immunoblot showing no change in expression of the LHCGR in whole brain lysates upon OVX or FSH-Ab treatment (N = 2 mice per group). k, IVIS imaging of isolated tissues from mice injected with AlexaFluor750–FSH, i.v., showing localization of FSH in the brain (N = 3 mice per group). l, Western immunoblots of whole brain lysates showing that i.p. injection of human FSH (5 IU) causes an elevation of brain FSH (N = 3 mice per group). m, Immunofluorescence micrographs showing the detection of peripherally injected (i.p.) biotinylated FSH-Ab (red) and biotinylated goat IgG (red) in brain sections (scale bar, 20 μm). Note the absence of cellular or nuclear co-localization with MAP2 or DAPI, respectively. n, Representative PET image shows that 89Zr-labelled humanized monoclonal FSH-Ab (89Zr-Hu6), injected i.v., is localized to live brain (arrows). γ-counting in perfused tissue shows presence of 89Zr-Hu6 in dissected brain tissue at 24 and 48 h post-injection (N = 4 mice). o, IVIS imaging and quantitation with AlexaFluor750-labelled Hu6, given i.v. shows localization in perfused whole brain tissue; N = 3 mice per group. Control (Ctrl): phosphate-buffered saline (PBS). p, Confirmatory immunofluorescence on the same mice (o) using anti-human IgG showing Hu6 localization (red) in proximity to CD31+ endothelial cells (green) (scale bar, 100 μm). For gel source data, see Supplementary Fig. 1.

Article Snippet: The human FSH ELISA kit was obtained from Abcam (ab108641).

Techniques: Immunofluorescence, TUNEL Assay, Staining, Transmission Assay, Transgenic Assay, Whisker Assay, Two Tailed Test, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Imaging, Isolation, Injection, Quantitation Assay, Control, Saline

Effects of platinum chemotherapeutic drugs on the levels of gonadotropin, melatonin, and oxidative stress in the peripheral blood of male gastrointestinal tumor patients, and reducing the expression of MT1/MT2 in the testis. ( A ) Human testicular tissue was stained with H&E (scale bar = 50 μm). ( B ) Images of TUNEL staining in human testicular tissue (scale bar = 50 μm). ( C ) Representative immunofluorescent pictures of MT1 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT1 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( D ) Representative immunofluorescent pictures of MT2 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT2 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( E ) MT1 mRNA expression in human testicular tissue. ( F ) MT2 mRNA expression in human testicular tissue. ( G ) GnRH level in human serum. ( H ) FSH level in human serum. ( I ) LH level in human serum. ( J ) Testosterone level in human serum. ( K ) Melatonin level in human serum. ( L ) Content of MDA in human serum. ( M ) SOD activity in human serum. ( N ) Total antioxidant capacity in human serum. Data are presented as the mean ± SEM. N = 3 cases per group in ( A – F ). N = 30 cases per group in ( G – N ). CT, chemotherapy. * p < 0.05, ** p < 0.01 versus the control group or the tumor group.

Journal: Cells

Article Title: Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway

doi: 10.3390/cells11101690

Figure Lengend Snippet: Effects of platinum chemotherapeutic drugs on the levels of gonadotropin, melatonin, and oxidative stress in the peripheral blood of male gastrointestinal tumor patients, and reducing the expression of MT1/MT2 in the testis. ( A ) Human testicular tissue was stained with H&E (scale bar = 50 μm). ( B ) Images of TUNEL staining in human testicular tissue (scale bar = 50 μm). ( C ) Representative immunofluorescent pictures of MT1 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT1 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( D ) Representative immunofluorescent pictures of MT2 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT2 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( E ) MT1 mRNA expression in human testicular tissue. ( F ) MT2 mRNA expression in human testicular tissue. ( G ) GnRH level in human serum. ( H ) FSH level in human serum. ( I ) LH level in human serum. ( J ) Testosterone level in human serum. ( K ) Melatonin level in human serum. ( L ) Content of MDA in human serum. ( M ) SOD activity in human serum. ( N ) Total antioxidant capacity in human serum. Data are presented as the mean ± SEM. N = 3 cases per group in ( A – F ). N = 30 cases per group in ( G – N ). CT, chemotherapy. * p < 0.05, ** p < 0.01 versus the control group or the tumor group.

Article Snippet: A Mouse MT (melatonin) ELISA Kit (Cat#: E-EL-M0788c), Human MT (melatonin) ELISA Kit (Cat#: E-EL-H2016c), GnRH (gonadotropin-releasing hormone) ELISA Kit (Cat#: E-EL-0071c), T (testosterone) ELISA Kit (Cat#: E-EL-0155c), Human FSH (follicle stimulating hormone) ELISA Kit (Cat#: E-EL-H1143c), and Human LH (luteinizing hormone) ELISA Kit (Cat#: E-EL-H6019) were purchased from Elabscience (Wuhan, China).

Techniques: Expressing, Staining, TUNEL Assay, Fluorescence, Labeling, Activity Assay, Control

Chemotherapy-induced premature ovarian insufficiency (POI) mice model. A Vaginal exfoliative cell smear and ovary section of normal mice (a–d, × 100; i, × 40; j, × 200) and POI mice (e–h, × 100; k, × 40, l, × 200). B Comparison of the follicles and the proportion of atretic follicles between CTX/BUS+ group and CTX/BUS− groups (* P < 0.05). C E2 and FSH levels determined by ELISA 14 days after CTX/BUS administration (* P < 0.05). E2 oestradiol, FSH follicle-stimulating hormone, CTX/BUS+ CTX/BUS administration, CTX/BUS− CTX/BUS non-administration

Journal: Stem Cell Research & Therapy

Article Title: Therapeutic effects of human umbilical cord mesenchymal stem cell-derived microvesicles on premature ovarian insufficiency in mice

doi: 10.1186/s13287-019-1327-5

Figure Lengend Snippet: Chemotherapy-induced premature ovarian insufficiency (POI) mice model. A Vaginal exfoliative cell smear and ovary section of normal mice (a–d, × 100; i, × 40; j, × 200) and POI mice (e–h, × 100; k, × 40, l, × 200). B Comparison of the follicles and the proportion of atretic follicles between CTX/BUS+ group and CTX/BUS− groups (* P < 0.05). C E2 and FSH levels determined by ELISA 14 days after CTX/BUS administration (* P < 0.05). E2 oestradiol, FSH follicle-stimulating hormone, CTX/BUS+ CTX/BUS administration, CTX/BUS− CTX/BUS non-administration

Article Snippet: The levels of estradiol (E2) and follicle-stimulating hormone (FSH) were measured with ELISA Kit (Cusabio, China).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay

Changes of hormone levels after HUCMSC-MVs transplantation. Serum levels of estradiol (E2) and follicle stimulating hormone (FSH) in three groups (* P < 0.05 vs control group, # P < 0.05 vs POI-MVs group

Journal: Stem Cell Research & Therapy

Article Title: Therapeutic effects of human umbilical cord mesenchymal stem cell-derived microvesicles on premature ovarian insufficiency in mice

doi: 10.1186/s13287-019-1327-5

Figure Lengend Snippet: Changes of hormone levels after HUCMSC-MVs transplantation. Serum levels of estradiol (E2) and follicle stimulating hormone (FSH) in three groups (* P < 0.05 vs control group, # P < 0.05 vs POI-MVs group

Article Snippet: The levels of estradiol (E2) and follicle-stimulating hormone (FSH) were measured with ELISA Kit (Cusabio, China).

Techniques: Transplantation Assay, Control

Regression analysis for conductometric technique and ELISA, correlation coefficient r2 is 0.703

Journal: The Malaysian Journal of Medical Sciences : MJMS

Article Title: The Use of Electronic Sensor in Hormone Analysis

doi:

Figure Lengend Snippet: Regression analysis for conductometric technique and ELISA, correlation coefficient r2 is 0.703

Article Snippet: Chemicals used include bovine serum albumine (BSA), Tween 20, Urease type VII, urea, human Folicle Stimulating Hormone (hFSH) from Sigma (USA), hFSH ELISA kit from Veda Lab (USA) and polyclonal antibody to FSH from the Endocrine Unit, Universiti Kebangsaan Malaysia (UKM).

Techniques: Enzyme-linked Immunosorbent Assay

Regression analysis of patient samples for conductometric technique and ELISA, correlation coefficient r2 is 0.894

Journal: The Malaysian Journal of Medical Sciences : MJMS

Article Title: The Use of Electronic Sensor in Hormone Analysis

doi:

Figure Lengend Snippet: Regression analysis of patient samples for conductometric technique and ELISA, correlation coefficient r2 is 0.894

Article Snippet: Chemicals used include bovine serum albumine (BSA), Tween 20, Urease type VII, urea, human Folicle Stimulating Hormone (hFSH) from Sigma (USA), hFSH ELISA kit from Veda Lab (USA) and polyclonal antibody to FSH from the Endocrine Unit, Universiti Kebangsaan Malaysia (UKM).

Techniques: Enzyme-linked Immunosorbent Assay